High Nutritional Quality of Human-Induced Pluripotent Stem Cell-Generated Proteins through an Advanced Scalable Peptide Hydrogel 3D Suspension System

Cell-cultured protein technology has become increasingly attractive due to its sustainability and climate benefits. The aim of this study is determine the nutritional quality human-induced pluripotent stem cell (hiPSC)-cultured proteins in an advanced 3D peptide hydrogel system for highly efficient production cell-cultured proteins. Our previous demonstrated a PGmatrix embedded culture long-term hiPSC maintenance expansion (PGmatrix-hiPSC (PG-3D)), which showed significantly superior pluripotency when compared with traditional 2D on Matrigel and/or Vitronectin other existing scaffolding systems such as Polyethylene glycol (PEG)-based hydrogels. In study, we designed suspension (PG-3DSUSP) from PG-3D that allows scaling up volume by 20 times (e.g., 0.5 mL 10 mL). results indicated PG-3DSUSP was competitive well-established method terms growth performance pluripotency. hiPSCs cultured consistently presented 15–20-fold increase 95–99% viability across multiple passages spheroids size range 30–50 μm. expression pluripotency-related genes, including NANOG, OCT4, hTERT, REX1, UTF1, PG-3DSUSP-cultured similar or higher than observed system, suggesting continuous maintenance. value hiPSC-generated further evaluated amino acid composition vitro digestibility. essential content (39.0%) human skeletal muscle (31.8%). digestibility (78.0 ± 0.7%) commercial beef isolate (75.7 0.6%). Taken together, first report produce possess more acids better nutrition may be particular significance novel alternative food engineering industries future food, beverage, supplement applications.